P. aeruginosa secretes a variety of 2-alkyl-4-quinolone (AQ) metabolites that mediate a broad range of virulence functions, including cell-to-cell communication, toxic activity against host cells, and polymicrobial interactions. Luke’s studies are focused on mass spectrometric analysis of Pseudomonas metabolites, and he is co-mentored by Dr. Maureen Kane in the Department of Pharmaceutical Sciences. One of Luke’s projects is aimed at determining the mechanism by which iron regulates AQ production, both during mono-culture and co-culture. He is also analyzing clinical CF samples for the presence of heme and AQ metabolites to determine if their presence correlates with disease exacerbation.
Publications:
Djapgne L, Panja S, Brewer LK, Gans J, Kane MA, Woodson SA, Oglesby-Sherrouse AG. (2018) The Pseudomonas aeruginosa PrrF1 and PrrF2 small regulatory RNAs (sRNAs) promote 2-alkyl-4-quinolone production through redundant regulation of the antR mRNA. Journal of Bacteriology. 200(10):e00704-17.
Huang W, Brewer LK, Jones JW, Nguyen AT, Marcu A, Wishart DS, Oglesby-Sherrouse AG, Kane MA, Wilks A. (2018) PAMDB: a comprehensive Pseudomonas aeruginosa metabolome database. Nucleic Acids Research 46(D1) D575–D580.
Reinhart AA, Nguyen AT, Brewer LK, Bevere J, Jones JW, Kane MA, Damron FH, Barbier M, and Oglesby-Sherrouse AG. (2017) The Pseudomonas aeruginosa PrrF small RNAs regulate iron homeostasis during acute murine lung infection. Infection and Immunity 85(5).
The Oglesby Lab 