Growth & Media
Basic Growth Media
DTSB Recipe
M9 Minimal Medium
CAS Agar for Pseudomonas aeruginosa
Deferrated EDDA
Care & handling of PAO1 fur mutants
Mini-Scale Pyoverdine Purification
Anaerobic Growth of Pseudomonas
Genetics
Boiling Miniprep
Transformation by Rb-Mn Method
E. coli CaCl competent cells protocol
E. coli Electroporation Protocol
Electrocompetent Pseudomonas
Mating Procedure for Pseudomonas
Complementation of Unmarked Pseudomonas Mutants
Isolation of chromosomal DNA
Gene Expression Assays
Complete real time PCR protocol
TaqMan Primer-Probe Design
Small RNA Northern Blot
Denaturing PAGE
Western Blot
Affinity Purification of Antibody
β-galactosidase Assay
Fur Mobility Shift Protocol
Sequence-Specific Affinity Chromatography
The Oglesby Lab 